Effect of oxygen concentration on singlet oxygen luminescence detection
| Autor: | Zhihai Qiu, Yirong Li, Buhong Li, Huiyun Lin, Lisheng Lin, Longchao Chen, Ying Gu |
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| Rok vydání: | 2014 |
| Předmět: |
Singlet oxygen
Biophysics Analytical chemistry General Chemistry Condensed Matter Physics Photochemistry Biochemistry Fluorescence Atomic and Molecular Physics and Optics chemistry.chemical_compound chemistry Rose bengal Photosensitizer Limiting oxygen concentration Irradiation Phosphorescence Luminescence |
| Zdroj: | Journal of Luminescence. 152:98-102 |
| ISSN: | 0022-2313 |
| DOI: | 10.1016/j.jlumin.2013.10.034 |
| Popis: | Singlet oxygen ( 1 O 2 ) is a major phototoxic component in photodynamic therapy (PDT) and its generation is dependent on the availability of tissue oxygen. To examine the effect of oxygen concentration on 1 O 2 detection, two hydrophilic photosensitizer (PS), rose bengal (RB) and meso-metra (N-methyl-4-pyridyl) porphine tetra tosylate (TMPyP) were used as model PS. Irradiation was carried out using 523 nm under hypoxic (2%, 13%), normoxic (21%) and hyperoxic (65%) conditions. The spectral and spatial resolved 1 O 2 luminescence was measured by near-infrared (NIR) photomultiplier tube (PMT) and camera, respectively. Upon the irradiation, the emission signal mainly consisted of background scattering light, PS fluorescence and phosphorescence, and 1 O 2 luminescence. The PS phosphorescence was evidently dependent on the oxygen concentration and PS type, which resulted in the change of emission profile of 1 O 2 luminescence. This change was further demonstrated on 1 O 2 luminescence image. The present study suggests that the low oxygen concentration could affect 1 O 2 luminescence detection. |
| Databáze: | OpenAIRE |
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