| Autor: |
Luca Montanarella, Sebastiano Di Biase, Anne-Francesca Lecoq |
| Rok vydání: |
1993 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography A. 638:363-373 |
| ISSN: |
0021-9673 |
| Popis: |
The possible use of capillary electrophoresis (CE) in micellar conditions with fast atom bombardment mass spectrometry (FAB-MS) for the characterization of DNA adducts with the ultimate goal of determining these compounds in biological matrices was explored. A method for fraction collection from an optimized and automated micellar electrokinetic capillary chromatographic (MECC) system is described. Parameters such as the reproducibility of migration times and injection and the maximum mass loadings are addressed. Fractions were collected directly in a small volume (5 μl) of buffer with sodium dodecyl sulphate (SDS) with recoveries of >75%. The fractions collected were further analysed using MECC and FAB-MS. Preliminary analysis by FAB-MS showed high background signals due to the presence of the SDS, demonstrating the difficulties that will be encountered with fractions deriving from a micellar separation and the need for more detailed investigations of the mass spectrometric conditions in this special case. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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