Macrophage Gene Therapy: opening novel therapeutic avenues for immune disorders
| Autor: | Preeti Bajaj, P. K. Singh, Chirag Chopra, U.C. Pachouri, Gyanesh Singh |
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| Rok vydání: | 2015 |
| Předmět: |
General Immunology and Microbiology
business.industry medicine.medical_treatment Genetic enhancement Promoter General Medicine Immunotherapy Transfection General Biochemistry Genetics and Molecular Biology Immune system Naked DNA medicine Cancer research Macrophage General Pharmacology Toxicology and Pharmaceutics business Gene Neuroscience |
| Zdroj: | F1000Research. 4:495 |
| ISSN: | 2046-1402 |
| DOI: | 10.12688/f1000research.6817.1 |
| Popis: | Macrophages are probably the most important cells of the mammalian immune system, and compromised macrophage function is known to cause several diseases. Their involvement in arthritis, cancer, infections, atherosclerosis, diabetes, and autoimmune disorders is well known. There has been a constantly growing need to transfer therapeutic genes into macrophages. Like most non-macrophage gene therapies,in vitrogene transfer has been attempted much more frequently in case of macrophages. However, primary macrophages are still somewhat recalcitrant to transfection. Macrophage-specific synthetic promoters, which were recently used successfully, can have up to 100-fold higher activity than that of native promoters. Adenovirus, lentivirus, and adeno-associated virus are commonly used for macrophage gene therapy. A number of non-viral methods are also popular for the transfer of exogenous DNA into macrophages. Gene transfer to macrophages using naked DNA has also been successful in a few cases. Macrophages have specific mechanisms to recognize and respond to bacterial DNA because of the presence of unmethylated CpG dinucleotides, which are rare in eukaryotic DNA. With interesting developments in this area, macrophage gene therapy appears to have great potential for immune therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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