Quantification of apolipoprotein A-IV in human plasma by immunonephelometry

Autor:	Armin Steinmetz, S Schwarz, C Luley, B Haas, J R Schäfer
Rok vydání:	1994
Předmět:	Reproducibility Chromatography Apolipoprotein B biology Chemistry Biochemistry (medical) Clinical Biochemistry Apolipoprotein A-IV Human plasma Blood plasma biology.protein Routine analysis Nephelometry Quantitative analysis (chemistry)
Zdroj:	Clinical Chemistry. 40:1717-1721
ISSN:	1530-8561 0009-9147
Popis:	We developed a nephelometric procedure for determining concentrations of human plasma apolipoprotein (apo) A-IV. Results obtained correlate well with those measured by an established electroimmunodiffusion assay (r = 0.98; n = 56). Intra- and interassay CVs were 2.4% and 2.0%, respectively, indicating excellent precision and reproducibility. Various conditions of sample treatment, such as adequate storage, freezing, and thawing, did not affect results significantly. However, keeping samples at room temperature for 4 days led to a slight increase in measured values. Preincubation with a cholesterinesterase-detergent mixture abolished interference from triglyceride-rich lipoproteins, allowing assay of samples containing triglycerides as great as 10 g/L. The assay is easily applicable to clinical laboratories for routine diagnostic use, as shown with hypertriglyceridemic plasmas and samples with a broad range of apo A-IV concentrations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::80737f39e404cced04e8288c26da164b https://doi.org/10.1093/clinchem/40.9.1717 Zobrazit plný text záznamu