A RADIOIMMUNOASSAY FOR OCHRATOXIN A: A PRELIMINARY INVESTIGATION

Autor:	B. Hald, O. Aalund, P. Krogh, K. Brunfeldt, K. Poulsen
Rok vydání:	2009
Předmět:	Ochratoxin A Detection limit biology Toxin food and beverages Radioimmunoassay General Medicine Secondary metabolite biology.organism_classification medicine.disease_cause chemistry.chemical_compound chemistry Biochemistry Antigen Penicillium medicine Ochratoxin medicine.drug
Zdroj:	Acta Pathologica Microbiologica Scandinavica Section C Immunology. :390-392
ISSN:	0304-1328
DOI:	10.1111/j.1699-0463.1975.tb01655.x
Popis:	Ochratoxin A is a nephrotoxic secondary metabolite of several fungal species included in the genera Aspergillus and Penicillium. A radioimmunoassay for this toxin is described. The reagents included antibodies against a coupling product of ochratoxin A and bovine IgG. 125I-labelled egg-albumin-ochratoxin A was employed as radioactive antigen. Free ochratoxin A dissolved in phosphate-buffered saline inhibited immunoprecipitation of the radioactive antigen. The procedure showed a lower limit of detection at 20 ppb of ochratoxin A.
Databáze:	OpenAIRE
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