Effect of human adipose tissue-derived mesenchymal-stem-cell bioactive materials on porcine embryo development
| Autor: | Dong-Sun Lee, Min-Jee Park, Eun-Young Kim, Key-Jung Riu, Hyo-Young Park, Seung-Eun Lee, Yeo-Jin Son, Jeremiah Jiman Moon, Jun Beom Lee, Chang-Jin Jeong, Hyun-Yong Choi, Se-Pill Park |
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| Rok vydání: | 2013 |
| Předmět: |
animal diseases
Embryogenesis Mesenchymal stem cell Adipose tissue Embryo Cell Biology Biology Fibroblast growth factor environment and public health Andrology enzymes and coenzymes (carbohydrates) Vascular endothelial growth factor A Cell culture parasitic diseases embryonic structures Immunology Genetics Fetal bovine serum Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 80:1035-1047 |
| ISSN: | 1040-452X |
| Popis: | Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) secrete bioactive materials that are beneficial for tissue repair and regeneration. In this study, we characterized human hAT-MSC bioactive material (hAT-MSC-BM), and examined the effect of hAT-MSC-BM on porcine embryo development. hAT-MSC-BM was enriched with several growth factors and cytokines, including fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA), and interleukin 6 (IL6). Among the various concentrations and days of treatment tested, 10% hAT-MSC-BM treatment beginning on culture Day 4 provided the best environment for the in vitro growth of parthenogenetic porcine embryos. While the addition of 10% fetal bovine serum (FBS) increased the hatching rate and the total cell number of parthenogenetic porcine embryos compared with the control and hAT-MSC culture medium group, the best results were from the group cultured with 10% hAT-MSC-BM. Mitochondrial activity was also higher in the 10% hAT-MSC-BM-treated group. Moreover, the relative mRNA expression levels of development and anti-apoptosis genes were significantly higher in the 10% hAT-MSC-BM-treated group than in control, hAT-MSC culture medium, or 10% FBS groups, whereas the transcript abundance of an apoptosis gene was slightly lower. Treatment with 10% hAT-MSC-BM starting on Day 4 also improved the development rate and the total cell number of in vitro-fertilized embryos. This is the first report on the benefits of hAT-MSC-BM in a porcine embryo in vitro culture system. We conclude that hAT-MSC-BM is a new, alternative supplement that can improve the development of porcine embryos during both parthenogenesis and fertilization in vitro. |
| Databáze: | OpenAIRE |
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