Human myelotoxicity of two phycotoxins, okadaic acid and domoic acid. Anin vitrostudy
| Autor: | Y. Sibiril, R. Froquet, Dominique Parent-Massin, P. Batina, N. Hymery |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Toxicological & Environmental Chemistry. 90:141-152 |
| ISSN: | 1029-0486 0277-2248 |
| DOI: | 10.1080/02772240701374948 |
| Popis: | Okadaic acid (OA), a diarrheic shellfish toxin and domoic acid (DA), an amnesic shellfish toxin are phycotoxins produced by various species of microalgaes. The aim of this study was to explore the effects of OA and DA on hematopoietic progenitors. For this purpose, three lineages of human hematopoietic progenitors, CFU-GM, BFU-E and CFU-MK optimized for toxicological studies were used. The effects of OA and DA on cell precursor proliferation and differentiation were investigated at concentration from 0.5 to 25 ng mL−1 and from 0.1 to 100 µg mL−1, respectively. A study of concentration and effect relationship showed a cytotoxic effect of OA (25 ng mL−1) for human hematopoietic progenitors. Moreover, this phycotoxin inhibited erythroid progenitors proliferation and disturbed differentiation at concentrations of 5 ng mL−1 and higher. OA IC50 were determined at 7.5 ng mL−1, 7.54 ng mL−1 and 1.19 ng mL−1 for CFU-GM, BFU-E and CFU-MK, respectively. Cytotoxic effects of OA and DA were compared to those effect of... |
| Databáze: | OpenAIRE |
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