Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents
| Autor: | Yuki Inoue, Hidenori Nagai, Natsuko Uchida, Shunsuke Furutani, Mizuho Murahashi, Tomohiko Ikeuchi, Satoshi Kondo, Yasuo Seto, Wilfred Espulgar, Eiichi Tamiya, Hirotaka Uzawa, Masato Saito, Naoki Nagatani |
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| Rok vydání: | 2018 |
| Předmět: |
Air sampling
education.field_of_study Computer science Materials Science (miscellaneous) 010401 analytical chemistry Population Nanotechnology 02 engineering and technology Pcr chip 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Biological terrorism Industrial and Manufacturing Engineering Atomic and Molecular Physics and Optics 0104 chemical sciences Microfluidic chip Toxic proteins Biological warfare Electrical and Electronic Engineering 0210 nano-technology education Biosensor |
| Zdroj: | Microsystems & Nanoengineering. 4 |
| ISSN: | 2055-7434 |
| DOI: | 10.1038/micronano.2017.83 |
| Popis: | The threat of biological and chemical terror acts remains a growing worldwide concern. There is therefore a need to develop appropriate technology for the detection of chemical and biological warfare agents, with early identification intended for use by first responders. Here, we disclose the developed autonomous air sampling and detection system for evaluation of the presence of chemical and biological warfare agents that can be harmful to the population. The current device utilizes the designed mist generator-assisted air collection system (338 l min−1) and biosensing chip technologies, such as electrochemical measurement, Au nanoparticle-based localized surface plasmon resonance, and rapid microfluidic chip PCR for detection of minute concentrations lower than the mean lethal dose (LD50) of nerve gases (sarin and VX), toxic proteins (BTX/A/Hc and ricin), and pathogens (anthrax simulant). An operation time of only 5–15 min is needed for the collection and detection; sample preparation is already integrated into the system without the need for direct human intervention. In addition to the system’s sensitivity and ease of use, its portability makes it highly beneficial for first responders, which could aid in immediate risk assessment and mitigation of on-site events. A portable device that can detect dangerous chemical and biological warfare agents at less than the mean lethal dose has been developed. Chemical and biological terrorism represents an increasing threat worldwide. Although various sensors have been developed for detecting toxins and pathogenic agents, there remains a need for a portable device that first responders can use to perform initial risk assessments. Masato Saito of Osaka University in Japan and co-workers have achieved this by combining three detection strategies in one device. The device samples air from the environment and then uses electrochemical measurements to detect nerve gases, gold nanoparticles to detect toxic proteins, and a PCR chip to detect pathogens. Molecules of interest can be detected in 5 to 15 minutes, and the device can be transported in a 30-centimeter cubic case. |
| Databáze: | OpenAIRE |
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