An additional dimer linkage structure in moloney murine leukemia virus RNA 1 1Edited by D. E. Draper

Autor:	Peter C.E Kang, Lori A Kohlstaedt, Emin Oroudjev
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Untranslated region Stereochemistry Dimer RNA Biology biology.organism_classification Molecular biology chemistry.chemical_compound Retrovirus chemistry Structural Biology Murine leukemia virus Nucleotide Guide RNA Nucleic acid structure Molecular Biology
Zdroj:	Journal of Molecular Biology. 291:603-613
ISSN:	0022-2836
Popis:	We have identified an additional dimerization linkage structure in the genome of Moloney murine leukemia virus (MoMLV). Retroviral genomes have long been known to be linked at their 5′ ends to form dimers. In MoMLV, a hairpin loop functioning as a dimer linkage structure (DLS) has previously been identified at nucleotides 278–303. Here, we describe RNA dimers formed from sections of the MoMLV 5′ untranslated region that do not contain the previously described MoMLV DLS. These dimers exhibit the distinctive characteristics previously described for whole genome dimers. We have mapped this novel region to nucleotides 199–243. This sequence contains a stem-loop structure (nucleotides 204–227) much like the 278–303 region. We describe the chemical and thermal stability of dimers containing the 204–227 stem-loop as well as kinetics and salt-dependence of dimer formation. Our results show that dimerization of MoMLV RNA can be nucleated at multiple sites and suggest that the 5′ untranslated region may contain separately folding and dimerizing domains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::805414195d90fd07c8ce38ebc5f57097 https://doi.org/10.1006/jmbi.1999.2984 Zobrazit plný text záznamu Full Text from ScienceDirect