Simulation of computational fluid dynamics and comparison of cephalosporin C fermentation performance with different impeller combinations

Autor: Hongzhen Luo, Yanli Zhao, Shengbing Duan, Fan Liu, Zhongping Shi, Guoqiang Yuan, Weijia Ni
Rok vydání: 2013
Předmět:
Zdroj: Korean Journal of Chemical Engineering. 30:1097-1104
ISSN: 1975-7220
0256-1115
Popis: Cephalosporin C (CPC) fermentation by Acremonium chrysogenum is an extremely high oxygen-consuming process and oxygen transfer rate in a bioreactor directly affects fermentation performance. In this study, fluid dynamics and oxygen transfer in a 7 L bioreactor with different impellers combinations were simulated by computational fluid dynamics (CFD) model. Based on the simulation results, two impeller combinations with higher oxygen transfer rate (K L a) were selected to conduct CPC fermentations, aiming at achieving high CPC concentration and low accumulation of major by-product, deacetoxycephalosporin (DAOC). It was found that an impeller combination with a higher K L a and moderate shear force is the prerequisite for efficient CPC production in a stirred bioreactor. The best impeller combination, which installed a six-bladed turbine and a four-pitched-blade turbine at bottom and upper layers but with a shortened impellers inter-distance, produced the highest CPC concentration of 35.77 g/L and lowest DAOC/CPC ratio of 0.5%.
Databáze: OpenAIRE
Externí odkaz: