Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme-Linked Immunosorbent Assay for PSPB and the Progesterone Assay
| Autor: | C.W. Passavant, T. A. Abdulkareem, S. M. Eidan, J.R. Branen, M A Alnimr, S.A.M. Al-Sharifi, R. G. Sasser, M.A. Ishak |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pregnancy Venipuncture Early pregnancy factor Elisa assay Plasma levels Biology medicine.disease biology.organism_classification Andrology Endocrinology Internal medicine Elisa test medicine biology.protein Animal Science and Zoology Plasma progesterone Bubalus Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 46:455-462 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/j.1439-0531.2010.01689.x |
| Popis: | Contents This study was undertaken to detect pregnancy in Iraqi riverine buffalo (Bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy-specific protein B (PSPB) with the BioPRYN® enzyme-linked immunosorbent assay (ELISA) test. The aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. Twenty-two female riverine buffalo that were 6.0 ± 0.93 years old were used. Four blood samples per buffalo were taken via jugular venipuncture at days 22–24, 32–34, 42–44 and 58–61 post-mating (PM) to measure the progesterone concentration (ng/ml) and to detect the presence of plasma PSPB. The rectal palpation method was employed to evaluate all buffalo on days 42–44 and 58–61 PM. The BioPRYN® test differed (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text