Practical Value of PGM 1 Subtyping in Blood- and Semen Stains and Hair Roots

Autor: E.-M. Hußlein, K.-U. Koch, J. Lötterle
Rok vydání: 1990
Předmět:
Zdroj: Advances in Forensic Haemogenetics ISBN: 9783540522713
DOI: 10.1007/978-3-642-75496-8_66
Popis: There is no doubt about the value of PGM 1 typing of bloodstains. The first investigations using starch gel electrophoresis were done by Culliford (1967), Heidel (1958) and Brinkmann (1969). The amount of stain needed was extremely large (between 10 and 50 mg of dried blood). With the introduction of agarose gel electrophoresis (Monn 1968; Culliford 1971; Grunbaum 1974) and cellulose acetate electrophoresis (Zajac and Sprague 1975) the quantity of stain could be reduced considerably. Isoelectric focusing allowed subtyping of PGM 1 of fresh blood and stains with a significantly higher value of information than before (Bark et al. 1976). Especially Berg et al. (1979, 1981), Schwerd and Hein (1983), Rittner et al. (1981), Oya et al. (1934) and Lawton and Kerr (1934) pointed out that additional or conversional bands occur with old blood samples respectively artificially prepared bloodstains as well as with cadaver tissue, thus leading to a false zymograms interpretation in some cases. Such conversional bands were not reported on in the few field studies published so far (blood- and seminal stains: Budowle et al. 1986; seminal stains: Oya et al. 1982). Therefore further examinations with practical cases seemed to be desirable.
Databáze: OpenAIRE