Reactive Oxygen Species and X-Ray Disrupted Spontaneous [Ca2+]iOscillation in Alveolar Macrophages

Autor:	Zhi-Xiong Long, Feng Chen, Hong Ye, Qiong Zhou, Peng-Cheng Cai, Jian-Bao Xin, Hao Chen, Yang Jin, Wan-Li Ma, Da-Quan Meng, Xiao-Nan Tao, Li-Ping Zhu, Qiu Tang
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Reactive oxygen species Radiation Biophysics Lung injury Fluorescence Molecular biology Tetrandrine Cytosol chemistry.chemical_compound chemistry Biochemistry In vivo Radiology Nuclear Medicine and imaging Irradiation Hydrogen peroxide
Zdroj:	Radiation Research. 179:485-492
ISSN:	1938-5404 0033-7587
DOI:	10.1667/rr3006.1
Popis:	Radiation leads to a rapid burst of reactive oxygen species (ROS), which is considered to be one of the major causes of radiation-induced injury. ROS have previously been shown to induce changes in cytosolic Ca2+ ([Ca2+]i) including [Ca2+]i oscillation. However, the role of radiation in [Ca2+]i oscillation is poorly understood. The purpose of this study was to identify the effect of ROS and X ray on [Ca2+]i oscillation, as well as their role in radiation-induced lung injury. Alveolar macrophages were cultured in the absence and presence of different doses of hydrogen peroxide (H2O2) or exposed to X-ray irradiation with or without pretreatment of diphenyleneiodonium chloride (DPI, an inhibitor of NADPH oxidases) or tetrandrine (TET, a calcium entry blocker) and cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2. Rat radiation lung injury was induced in vivo by using 40 Gy X ray and DPI or TET was used to prevent radiation-induced lung injury. The results showed that there was sp...
Databáze:	OpenAIRE
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