Radioimmunoassay of Drugs Subject to Abuse: Critical Evaluation of Urinary Morphine—Barbiturate, Morphine, Barbiturate, and Amphetamine Assays
| Autor: | Salvatore J. Mulé, D Jukofsky, Eileen G. Whitlock |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Enzyme multiplied immunoassay technique
Drug Chromatography medicine.diagnostic_test Chemistry medicine.drug_class media_common.quotation_subject Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Liter Urine Pharmacology Barbiturate medicine Morphine Amphetamine medicine.drug media_common |
| Zdroj: | Clinical Chemistry. 21:81-86 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/21.1.81 |
| Popis: | Radioimmunoassays for morphine—barbiturate (MOR-BARB), morphine, barbiturate, and amphetamine were evaluated by a direct comparison with differential elution extraction thin-layer chromatography, the "enzyme multiplied immunoassay technique," and XAD-2 resin extraction thin-layer chromatography for the detection in urine of drugs subject to abuse. Statistically significant (P < 0.01) concentrations for detection were: 50-100 µg/liter for MOR-BARB; 5 µg/liter for morphine, 10 µg/liter for barbiturate, and 500 µg/liter for amphetamine. Unconfirmed and unaccounted-for radioimmunoassay positives (false) were: 0% for morphine in the radioimmunoassay for MOR-BARB and that for morphine alone; 2.8% for barbiturates in the MOR-BARB assay and that for barbiturates alone; 0-6% when a combination of these drugs was present in the MOR-BARB, morphine, or barbiturate assay; and 2.4% in the amphetamine radioimmunoassay. Less than 1% of all radioimmunoassay-negative samples were unconfirmed (false). Cross-reactivity was observed with drugs of a similar chemical structure in each of the radioimmunoassays tested. All the radioimmunoassays were easy to use, highly sensitive, and extremely reliable for detecting drug use or abuse. |
| Databáze: | OpenAIRE |
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