Synergistic assembly of peptide amphiphiles with varying polarities for encapsulation of camptothecin
| Autor: | Steffie Mano, Yen Wah Tong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
010302 applied physics
chemistry.chemical_classification Materials science Hydrogen bond Peptide 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry digestive system diseases chemistry Nanofiber 0103 physical sciences Amphiphile medicine Molecule heterocyclic compounds General Materials Science 0210 nano-technology neoplasms Protein secondary structure Peptide sequence Camptothecin medicine.drug |
| Zdroj: | Materialia. 8:100516 |
| ISSN: | 2589-1529 |
| DOI: | 10.1016/j.mtla.2019.100516 |
| Popis: | Peptide amphiphiles (PA) are a group of peptide-based biomaterial design inspired by lipid molecules in the cell membrane. They possess distinct hydrophobic and hydrophilic regions which can self-assemble into highly ordered nanostructures based on the peptide sequence. In this work, the design strategy employed, explores the innate ability of PA to serve as a depository for hydrophobic drug camptothecin (CPT). CPT was encapsulated into two oppositely charged PA by hydrophobic attraction which develops into hybrid nanofibers by the interplay of electrostatic interactions and hydrogen bonding. This method of encapsulation averts the use of physical and chemicals factors like pH, temperature and ions to enable the self-assembly process. The CPT encapsulated PA nanofiber (PA-CPT) structure exhibits a high encapsulation efficiency of 84.83 ± 4.12%. After encapsulation, it was observed that the secondary structure of PA and active lactone conformation of CPT were perfectly retained. In vitro release studies reveal that 40% of the drug was released in the first 12 h. PA-CPT was effectively taken up by HepG2 cells with a lower IC50 value of 0.56 µM/L compared to free CPT having an IC50 of 3.75 µM/L. The developed PA-drug system enhances the bioavailability of CPT in an aqueous environment. |
| Databáze: | OpenAIRE |
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