ORIGINAL ARTICLE: Expression of the Small Heat Shock Protein alphaB-Crystallin in Term Human Placenta
| Autor: | Wolfgang Gartner, Ivelina Mineva, Maria Stamenova, Ludwig Wagner |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Regulation of gene expression
medicine.diagnostic_test biology Immunology Obstetrics and Gynecology Immunofluorescence Molecular biology Syncytiotrophoblast medicine.anatomical_structure Reproductive Medicine Hsp27 Heat shock protein Placenta medicine biology.protein Immunology and Allergy Decidual cells sense organs Transcription factor |
| Zdroj: | American Journal of Reproductive Immunology. 60:440-448 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.2008.00642.x |
| Popis: | Problem Expression of heat shock proteins has been described in different tissues relevant to human reproduction, including placenta. AlphaB-crystallin is a member of the small heat shock protein family (sHsp) exerting biologically important chaperon functions. Method of study Immunofluorescence; immunoblot analysis; quantitative real-time-PCR; CpG island methylation analysis. Results In this study, we once again describe the expression of alphaB-crystallin in the stroma of the placental villi and in the cytoplasm of decidual cells by immunofluorescence. In contrast, Hsp27 – another sHsp family member – was detected exclusively in the syncytiotrophoblast layer. This varying expression pattern provides additional support to earlier reports of functional differences between both proteins. Semi-quantitative immunoblot analysis of placenta tissue specimens (n = 6) revealed Hsp27 expression exceeding that of alphaB-crystallin, albeit with inter-individual variations. Inter-individual alphaB-crystallin expression variations were confirmed by quantitative RT-PCR. CpG island methylation was ruled out as the underlying cause for the inter-individual alphaB-crystallin expression variations. However, the expression extent of GATA3, which is a transcription factor with corresponding elements within the alphaB-crystallin gene (CRYAB) promoter, paralleled that of alphaB-crystallin. We demonstrated remarkable GATA3 expression in placental tissue, exceeding that of other endocrine organs. Conclusion We can conclude that the differential expression patterns of alphaB-crystallin and Hsp27 indicate functional differences between these highly related proteins in placental tissues. |
| Databáze: | OpenAIRE |
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