A capillary electrophoresis method to explore the self-assembly of a novel polypeptide ligand with quantum dots
Autor: | Pengju Jiang, Liqian Gao, Lin Qiu, Jianhao Wang, Minli Fu, Ding Shumin, Li Liu, Karunakaran A. Kalesh, Zhang Chencheng |
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Rok vydání: | 2016 |
Předmět: |
chemistry.chemical_classification
Chemistry Clinical Biochemistry technology industry and agriculture Analytical chemistry Peptide 02 engineering and technology equipment and supplies 010402 general chemistry 021001 nanoscience & nanotechnology Ligand (biochemistry) 01 natural sciences Biochemistry Combinatorial chemistry 0104 chemical sciences Analytical Chemistry Capillary electrophoresis Förster resonance energy transfer Quantum dot Self-assembly 0210 nano-technology Peptide sequence Histidine |
Zdroj: | ELECTROPHORESIS. 37:2156-2162 |
ISSN: | 0173-0835 |
Popis: | Polyhistidine peptides are effective ligands to coat quantum dots (QDs). It is known that both the number of histidine (His) residues repeats and their structural arrangements in a peptide ligand play important roles in the assembly of the peptide onto CdSe/ZnS QDs. However, due to steric hindrance, a peptide sequence with more than six His residue tandem repeats would hardly coordinate well with Zn(2+) in the QD shell to further enhance the binding affinity. To solve this problem, a His-containing peptide ligand, ATTO 590-E2 G (NH)6 (ATTO-NH), was specifically designed and synthesized for assembly with QDs. With sequential injection of QDs and ATTO-NH into the capillary electrophoresis with fluorescence detection, strong Forster resonance energy transfer phenomenon between the QDs and the ATTO 590 dye was observed, indicating efficient self-assembly of the novel peptide onto the QDs to form ATTO-NH capped QDs inside the capillary. The binding stability of the ligand onto the QD was then systematically investigated by titrating with imidazole, His, and a his-tag containing competitive peptide. It is believed that this new in-capillary assay significantly reduced the sample consumption and the analysis time. By functionalizing QDs with certain metal cation-specific group fused peptide ligand, the QD-based probes could be even extended to the online detection of metal cations for monitoring environment in the future. |
Databáze: | OpenAIRE |
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