Autor:	Claudio Montero, Manuel Gutierrez, Erika Chi-Ahumada, Susana Chavez-Porras
Rok vydání:	2000
Předmět:	chemistry.chemical_classification Molar concentration biology medicine.drug_class Globular protein Cell Biology Monoclonal antibody Molecular biology Divalent chemistry.chemical_compound Antigen retrieval chemistry Biochemistry Antigen biology.protein medicine Immunohistochemistry Anatomy Antibody
Zdroj:	Histochemical Journal. 32:111-114
ISSN:	0018-2214
Popis:	Previously we have applied Scott's critical molar concentration concept to show that divalent cations, especially Mg2+ may be used to measure the affinity of a known monoclonal antibody for its antigen. In this paper we report the application of this same procedure to a study of a series of antigens (three globular proteins and three intermediate filaments). The concept was applied to samples without any previous treatment or after the application of heat-mediated antigen retrieval (using a pressure cooker). Our findings suggest that heat-mediated antigen retrieval sets free protein side-chain(s) that have been masked by formaldehyde fixation. This is reflected in a higher affinity of the antibody for the antigen in question.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::80132f1076d9e8b94cd59782126ddc74 https://doi.org/10.1023/a:1004070329155 Zobrazit plný text záznamu Full text from SpringerLink