Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Autor:	Mutsuo Tanaka, Osamu Niwa, Dai Kato, Tomoyuki Kamata, Masami Todokoro, Atsumu Oda, Yasuo Yoshimi, Seiichiro Iijima
Rok vydání:	2014
Předmět:	Lysine Analytical chemistry Electrochemistry Analytical Chemistry Low noise Ion chemistry.chemical_compound Adsorption Ferrocene chemistry Electrode medicine lipids (amino acids peptides and proteins) Polymyxin B Nuclear chemistry medicine.drug
Zdroj:	Electroanalysis. 26:618-624
ISSN:	1040-0397
Popis:	We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly-e-lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS-PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0 ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8012020b2de8b862bdfb0f3e1a74cdc5 https://doi.org/10.1002/elan.201300542 Zobrazit plný text záznamu Plný text ve formátu PDF