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The benefits of intensive insulin therapy for type 2 diabetics with cardiovascular disease (CVD) are highly controversial. We performed in vitro studies to determine whether insulin has any effect on whole blood coagulation and assess how insulin responds to monocyte membrane associated tissue factor (mmaTF) induced hypercoagulability. Mercuric ion (Hg/sup 2+/), which has been shown to increase mmaTF-dependent procoagulant activity in whole blood, was used to stimulate similar pathways in our experiment. We spiked human citrated whole blood with HgCl/sub 2/ and Humulin-N/sup /spl reg// (HN) at final concentrations of control, 0.02 U/mL HN, 0.004 U/mL HN, 0.002 U/mL HN, 0.005% HgCl/sub 2/, 0.02 U/mL HN:0.005% HgCl/sub 2/, 0.004 U/mL HN:0.005% HgCl/sub 2/, and 0.002 U/mL HN:0.005% HgCl/sub 2/. The samples (n=12) were incubated at 37/spl deg/C for 10 minutes and recalcified with CaCl/sub 2/ to initiate clotting. The clotting time (CT) was measured with an Amelung KC4A Micro. The results indicate that HN by itself has no effect on CT. However, each concentration of HN:HgCl/sub 2/ exhibited a shorter CT than either HN or HgCl/sub 2/ alone (p |
