Seeing through the interface: poly(ε -Caprolactone) surface modification of poly(glycerol-co-sebacic acid) membranes in adult porcine retinal explants
| Autor: | Fredrik Ghosh, Karin Arnér, Robert Langer, Linnéa Taylor, Gillian Hendy, Martin E. Kolewe, Christopher D. Pritchard |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Retina Sebacic acid Eosin Chemistry Biomedical Engineering Medicine (miscellaneous) Retinal Adhesion Haematoxylin Biomaterials Transplantation 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Membrane medicine.anatomical_structure 030221 ophthalmology & optometry medicine sense organs Biomedical engineering |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 11:2349-2358 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.2135 |
| Popis: | The purpose of this study was to investigate the adhesion properties and tissue reactions in an in vitro model of nanofabricated membranes emulating the vitreous cortex. Electrospinning was performed for either 5, 10 or 15 min to create various thicknesses of poly(e-caprolactone) (PCL) fibre mats on a poly(glycerol-co-sebacic acid) (PGS) surface. These were fused with adult porcine retinal explants, with the fibre side facing the inner retina, and cultured for 5 days. Adherence was assessed by macroscopic inspection, and morphological and immunohistochemical analysis was performed using haematoxylin and eosin (HE 15 min composite explants adhered only at focal points, were thin and showed extensive degenerative damage. The physical composition of nanofibre meshes is important for adhesion to the inner retina and has a significant impact on neuronal and glial survival in vitro. The results bearing on research involving retinal transplantation are discussed. (Less) |
| Databáze: | OpenAIRE |
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