Differential expression of immune‐related genes in Pacific white shrimp, Litopenaeus vannamei, previously reared in biofloc and challenged with Vibrio harveyi
| Autor: | Karla-Susana Escalante-Herrera, Edlin Guerra-Castro, Gabriela Rodríguez-Fuentes, Gabriela Gaxiola, Alejandra Prieto-Davó, Diana Aguilera-Rivera |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Vibrio harveyi Litopenaeus 04 agricultural and veterinary sciences Prophenoloxidase Aquatic Science Plant disease resistance biology.organism_classification Vibrio Microbiology Shrimp 03 medical and health sciences 040102 fisheries 0401 agriculture forestry and fisheries Pathogen Bacteria 030304 developmental biology |
| Zdroj: | Aquaculture Research. 50:2039-2046 |
| ISSN: | 1365-2109 1355-557X |
| DOI: | 10.1111/are.14063 |
| Popis: | This study aimed to evaluate possible modification of some immune‐related genes (hemocyanin, alpha2‐macroglobulin, prophenoloxidase and penaeidin3a) at the transcriptional level and signs of disease and mortality in Litopenaeus vannamei previously reared in biofloc and challenged with different Vibrio harveyi densities (10³–10⁸ CFU per ml). Gene expression was up‐ or downregulated based on bacterial density, indicating that defence mechanisms were activated to remove the pathogen. The genes analysed were not affected in the control group (without bacteria) or in the 10³ CFU per ml group. The prophenoloxidase gene was expressed in the 10⁴ CFU per ml group, whereas transcripts of the alpha2‐macroglobulin gene were detected in the 10⁵ and 10⁶ CFU per ml groups. However, the penaeidin3a and hemocyanin genes were upregulated in the 10⁷ and 10⁸ CFU per ml groups, in which signs of disease and mortality in shrimp were evident. These results suggest that the genes involved in the shrimp immune response fluctuate according to pathogen density, and when this density increases (e.g. 10⁷ and 10⁸ CFU per ml), the activated disease tolerance and defence mechanisms are inefficient. For this reason, disease and mortality were observed in the shrimp regardless of the previous condition of the biofloc. |
| Databáze: | OpenAIRE |
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