Essential role of K+uptake permease (Kup) for resistance to sucrose-induced stress inGluconacetobacter diazotrophicusPAl 5
| Autor: | Marcos V. V. de Oliveira, Leandro Fernandes Andrade, Aline Chaves Intorne, Gonçalo Apolinário de Souza Filho, Luciano de Souza Vespoli, Patrícia Louzada Rangel, Leandro de Mattos Pereira |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Sucrose Permease Mutant Wild type Mutagenesis (molecular biology technique) Biology biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Microbiology Complementation 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Gene Ecology Evolution Behavior and Systematics Bacteria |
| Zdroj: | Environmental Microbiology Reports. 9:85-90 |
| ISSN: | 1758-2229 |
| Popis: | Microorganisms are constantly challenged by stressful conditions, such as sugar-rich environments. Such environments can cause an imbalance of biochemical activities and compromise cell multiplication. Gluconacetobacter diazotrophicus PAl 5 is among the most sugar-tolerant bacteria, capable of growing in the presence of up to 876 mM sucrose. However, the molecular mechanisms involved in its response to high sucrose remain unknown. The present work aimed to identify sucrose-induced stress resistance genes in G. diazotrophicus PAl 5. Screening of a Tn5 transposon insertion library identified a mutant that was severely compromised in its resistance to high sucrose concentrations. Molecular characterization revealed that the mutation affected the kupA gene, which encodes a K+ uptake transporter (KupA). Functional complementation of the mutant with the wild type kupA gene recovered the sucrose-induced stress resistance phenotype. High sucrose resistance assay, under different potassium concentrations, revealed that KupA acts as a high-affinity K+ transporter, which is essential for resistance to sucrose-induced stress, when extracellular potassium levels are low. This study is the first to show the essential role of the KupA protein for resistance to sucrose-induced stress in bacteria by acting as a high-affinity potassium transporter in G. diazotrophicus PAl 5. |
| Databáze: | OpenAIRE |
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