An activatable fluorescent probe with an ultrafast response and large Stokes shift for live cell bioimaging of hypochlorous acid
| Autor: | Yong-Qing Kuang, Ying Tang, Jian-Hui Jiang, Ru-Qin Yu, Dan Pan, Feng Liu, Xian-Jun Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Detection limit Fluorophore Hypochlorous acid 010405 organic chemistry General Chemical Engineering Analytical chemistry General Chemistry 010402 general chemistry 01 natural sciences Aldehyde Fluorescence 0104 chemical sciences chemistry.chemical_compound symbols.namesake chemistry Intramolecular force Stokes shift symbols Biophysics Selectivity |
| Zdroj: | RSC Advances. 6:107910-107915 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra22686h |
| Popis: | A novel activatable fluorescent probe for hypochlorous acid (HOCl) imaging, has been developed based on HOCl-triggered aldehyde recovery reaction. This probe features with a long emission wavelength because of the use of intramolecular charge transfer (ICT) effect in the fluorophore. Moreover, this probe displayed ultrafast response (within 1 s), good selectivity and high sensitivity (detection limit of 50 nm) toward HOCl with excellent independency upon pH of the assay system. Live cells imaging studies demonstrate that the probe can be applied successfully to detect exogenous and endogenous HOCl. |
| Databáze: | OpenAIRE |
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