Vancomycin added to the wash solution of the cell-saver. Effect on bacterial contamination
| Autor: | E. Gredilla-Díaz, F. Gilsanz-Rodríguez, A. Perez-Ferrer, J. de Vicente-Sánchez, R. Navarro-Suay |
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| Rok vydání: | 2017 |
| Předmět: |
Blood transfusion
medicine.diagnostic_test medicine.drug_class Intraoperative blood salvage business.industry medicine.medical_treatment Antibiotics 030208 emergency & critical care medicine General Medicine 030204 cardiovascular system & hematology medicine.disease_cause 03 medical and health sciences Red blood cell 0302 clinical medicine medicine.anatomical_structure Anesthesia medicine Vancomycin Blood culture business Saline Staphylococcus medicine.drug |
| Zdroj: | Revista Española de Anestesiología y Reanimación (English Edition). 64:185-191 |
| ISSN: | 2341-1929 |
| DOI: | 10.1016/j.redare.2016.10.001 |
| Popis: | Objectives The aim of this study is to test whether the addition of a low-dose of antibiotic (vancomycin) to the wash solution (saline) of the cell-saver reduces the incidence of bacterial contamination of the autologous red blood cell (RBCs) concentrate recovered. Material and method Experimental, randomized, double-blind, parallel group study performed on 20 consecutive patients scheduled for posterior spinal fusion surgery. Intraoperative bleeding was processed through a cell-saver: HaemoLite ® 2+, in which the RBCs were washed according to randomization group, with saline (control group) or saline + 10 μg ml −1 vancomycin (vanco group). Data regarding age, weight, processed and recovered volume, blood count, blood culture, and vancomycin concentration in RBCs concentrates obtained and incidence of fever after reinfusion were collected. Results Processed volume was 843 ± 403 ml and recovered volume 121 ± 29 ml, with haemoglobin concentration 10.4 ± 5.0 g dl −1 and haematocrit 29.1 ± 15.9% (mean ± SD). Recovered RBC concentrate cultures were positive for coagulase-negative Staphylococcus in 5 cases (50%) of the control group while all cultures were negative in the vanco group ( p = 0.016). The difference between the theoretical concentration of vancomycin administered and the concentration determined in the recovered RBC concentrate was 1.31 μg ml −1 (95% CI 1.19–1.43; p = 0.074). Conclusions The addition of vancomycin at a concentration of 10 μg ml −1 to the wash solution of the cell-saver achieved similar concentrations in the autologous blood concentrate recovered allowing for bacterial removal, with negative blood cultures in all cases. |
| Databáze: | OpenAIRE |
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