Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)

Autor:	David Rickwood, J. M. Graham
Rok vydání:	2004
Předmět:	chemistry.chemical_classification Pathology medicine.medical_specialty Chemistry Diatrizoate Sedimentation Polysaccharide Iodixanol Molecular biology Peripheral blood mononuclear cell Peripheral blood Blood cell medicine.anatomical_structure medicine Cell separation medicine.drug
Zdroj:	eLS
DOI:	10.1038/npg.els.0003801
Popis:	Mononuclear cells (lymphocytes and monocytes) can be isolated from other blood cell elements on the basis of their lower density. There are three variations of the basic method: (1) sedimentation on to a density barrier using diatrizoate (plus a polysaccharide) or Nycodenz or iodixanol; (2) flotation after raising the density of the plasma by mixing blood with Nycodenz or iodixanol; (3) flotation through an iodixanol density barrier. Keywords: peripheral blood; human; lymphocytes; monocytes; mononuclear cell separation; cell separation
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7fd9ce6b4a5bd0006e6c34ade3c766bf https://doi.org/10.1038/npg.els.0003801 Zobrazit plný text záznamu