Effects of calorie-restriction on the course of Trypanosoma cruzi infection
| Autor: | A.R. Riarte, M.A. Carlomagno, E.L. Segura, M. Moreno |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Nutrition and Dietetics
biology Endocrinology Diabetes and Metabolism Calorie restriction Spleen Parasitemia medicine.disease biology.organism_classification Chronic infection Endocrinology Immune system medicine.anatomical_structure Delayed hypersensitivity Immunology medicine biology.protein Antibody Trypanosoma cruzi |
| Zdroj: | Nutrition Research. 7:1031-1040 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/s0271-5317(87)80174-4 |
| Popis: | The effect of moderately and severely caloriedeficient diets on the course of Trypanosoma cruzi inreceiving different daily amounts of laboratory chow and water ad libitum , were inoculated with 50 or 500 blood trypomastigotes (T). Onset of parasitemia was seen earlier in severely and moderately malnourished mice with respect to controls and survival time was significantly shorter for severely malnourished animals regardless of the infecting dose. All moderately malnourished animals died as well as control animals infected with 500 T, at different intervals, while 33% of those controls infected with 50 T survived the infection. All uninfected animals survived until sacrifice on day 50 after initiation of diet. Another set of animals, receiving 4 or 6 g/day/animal of laboratory chow, was infected with approximately 15 parasites per animal 2 weeks after initiation of diet to induce a chronic infection. Mice were sacrificed 4, 7, and 11 months post infection, and, delayed hypersensitivity (DTH), serum antibodies, spleen cells blastogenesis, and histopathology of several tissues were analyzed. Malnourished animals showed impaired DTH and lower circulating antibody levels as measured by ELISA. There was a significant correlation between pathological damage and degree of parasitemia reached during the acute phase of infection. Mitogenic response to Concanavalin A was not affected by the diet but increased as time progressed. These results show that severe caloric deficiencies significantly accelerate death due to infection when high doses of parasites are used. During chronic infection, moderate malnutrition impaired some aspects of the immune response, i.e., DTH and circulating IgG antibodies measured by ELISA, while mitogenic activity remained unchanged. Histopathological alterations were not dependent on the diet but correlated with the peak of parasitemia reached during the acute phase of the disease. |
| Databáze: | OpenAIRE |
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