Abstract 3765: High-throughput RNAi screening identifies genes controlling glioblastoma stem cell migration and survival

Autor:	Yue Liu, Jimmy C. Menjivar, Linda M. Liau, Yibei Zhang, William H. McBride, Cho-Lea Tso, Jane Y. Tian, Jonathan L. Tso
Rok vydání:	2013
Předmět:	Cancer Research education.field_of_study Cell migration Biology Bioinformatics Embryonic stem cell Autologous tumor cell Neural stem cell Endothelin 3 Oncology RNA interference Cancer research Stem cell Neural crest cell migration education
Zdroj:	Cancer Research. 73:3765-3765
ISSN:	1538-7445 0008-5472
DOI:	10.1158/1538-7445.am2013-3765
Popis:	Glioblastoma is incurable. Glioblastoma stem cells (GSC) became a model for explaining tumor recurrence due to their tumorigenic capacity, migratory nature, and radio-chemoresistant phenotype. To prevent tumor recurrence, a strategy targeting of GSC must be identified and added into the treatment. To identify vital genes that promote GSC migration and sustain self-renewal and anti-apoptotic features we have established tumorigenic CD133+ GSC lines (n=3 patients), which are capable of clonal self-renewal and asymmetric division for producing fast-growing CD133- daughter cells that form tumor spheres. CD133+ GSC can migrate outward from primary spheres and form spheres again, which provide an visible, functional model for identifying essential genes of CD133+GSC via loss-of-function RNA interference (RNAi) screen. To identify genes and pathways that confer the migration nature and super longevity of GSC, we constructed a siRNA library consisting of siRNA clones targeting 2079 genes overexpressed in tumorigenic CD133+ GSC when compared to non-tumorigenic, autologous tumor cell lines, normal brain tissue, human embryonic stem cells, normal neural stem cells, fetal neural progenitor cells and glioblastoma tumors, respectively, as determined by the comparative expression microarray analysis (E/B>2X, E-B>100, p value Citation Format: Yue Liu, Yibei Zhang, Jonathan L. Tso, Jimmy C. Menjivar, Jane Y. Tian, Linda Liau, William McBride, Cho-Lea Tso. High-throughput RNAi screening identifies genes controlling glioblastoma stem cell migration and survival. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3765. doi:10.1158/1538-7445.AM2013-3765
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7fcf5bba5fc865ca4d4c4e77691a77cd https://doi.org/10.1158/1538-7445.am2013-3765 Zobrazit plný text záznamu