NPY-mRFP Rat Basophilic Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation
| Autor: | Daniel Wan, Nafal J S Barwary, Franco H. Falcone |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biology Chemistry Degranulation Basophil Neuropeptide Y receptor Immunoglobulin E Fluorescence humanities Green fluorescent protein Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030228 respiratory system Cell culture medicine biology.protein Incubation |
| Zdroj: | Basophils and Mast Cells ISBN: 9781071606957 |
| DOI: | 10.1007/978-1-0716-0696-4_13 |
| Popis: | Humanized rat basophilic leukemia (RBL) reporter cell lines are increasingly used for the detection of allergen-specific IgE and other purposes, such as the detection of allergens and standardization of allergen preparations. Existing reporter systems have many strengths and advantages but can be expensive or require longer incubation times. The new NPY-mRFP reporter cell line addresses such problems, as it requires neither expensive substrates nor overnight incubation for detection of activation. The fusion of Neuropeptide Y (NPY) with monomeric Red Fluorescent Protein (mRFP) results in localization of the fluorescent protein in granules. As NPY-mRFP is preformed in granules, the reporter system activation can be assessed using fluorescence measurements after as soon as 45-60 min, as described in this chapter, without the need to add any substrates. |
| Databáze: | OpenAIRE |
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