Genetically Engineered Escherichia coli FBR5 to Use Cellulosic Sugars: Production of Ethanol from Corn Fiber Hydrolyzate Employing Commercial Nutrient Medium
| Autor: | Qureshi, N., Dien, B. S., Saha, B. C., Iten, L., Liu, S., Hughes, S. R. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| DOI: | 10.17628/ecb.2015.4.130-134 |
| Popis: | Transportation biofuel ethanol was produced from xylose and corn fiber hydrolyzate (CFH) in a batch reactor employing Escherichia coli FBR5. This strain was previously developed in our laboratory to use cellulosic sugars. The culture can produce up to 49.32 gL-1 ethanol from approximately 125 gL-1 xylose. Use of commercial nutrient sources such as corn steep liquor (CSL) and soy peptone (SP) was also studied and SP was found to be superior than CSL. SP at a concentration of 15 gL-1 resulted in the production of 42.2 gL-1 ethanol with ethanol yield and productivity of 0.49 and 0.74 gL-1h-1, respectively. Corn fiber (CF) was pretreated with dilute H2SO4 and hydrolyzed using commercial cellulases. Employing CFH as a sole substrate, the culture produced 35.33 gL-1 ethanol with a productivity and yield of 1.01 gL-1h-1 and 0.54, respectively, leaving behind no residual sugars in the medium. This productivity is 40% higher than when using xylose as the carbon source in the control experiment. European Chemical Bulletin, Vol 4, No 1-3 (2015): European Chemical Bulletin |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|