Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals
| Autor: | Sonja Krieger, Sonja Schneider, Edgar Naegele |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Wax Glycan Chromatography Glycosylation Ion exchange biology Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound Dimension (vector space) Two-dimensional chromatography visual_art visual_art.visual_art_medium biology.protein 030304 developmental biology Glycan Analysis |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071612408 |
| Popis: | EPO has a complex glycosylation pattern with differently branched and charged glycans. A combination of hydrophilic interaction chromatography (HILIC) with weak anion exchange chromatography (WAX) enables highly orthogonal separation. Comprehensive 2D-LC analysis with HILIC in the first and WAX in the second dimension provides high resolution 2D chromatography together with simultaneous charge profiling. Meanwhile, multiple heart-cutting 2D-LC analysis combining WAX and HILIC separation provides a flexible alternative whereby the user can select multiple peaks to be analyzed in the second dimension and, moreover, run longer gradients in the second dimension. |
| Databáze: | OpenAIRE |
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