Temperature Effects Of Excitation Laser Pulses During Step-Scan FT-IR Measurements

Autor:	H. Georg, C. Rödig, M. Sheves, I. Rousso, Friedrich Siebert
Rok vydání:	1999
Předmět:	biology Pulse (signal processing) Analytical chemistry Retinal Bacteriorhodopsin Chromophore Laser Biochemistry Atomic and Molecular Physics and Optics Spectral line law.invention chemistry.chemical_compound chemistry law biology.protein Temperature difference Spectroscopy Excitation
Zdroj:	Laser Chemistry. 19:169-172
ISSN:	1476-3516 0278-6273
DOI:	10.1155/1999/50762
Popis:	Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7f97af44926db8158d34b0e50551e1b4 https://doi.org/10.1155/1999/50762 Zobrazit plný text záznamu Plný text