Detecting Alaria esculenta and Laminaria digitata (Laminariales, Phaeophyceae) gametophytes in red algae, with consideration of distribution patterns in the intertidal zone
| Autor: | Alexander D. McKenzie, Simon J. Greenough, Emma S. Bergeron, Katherine S.A. Cripps, Trevor T. Bringloe, Gary W. Saunders, Alexa M.E. Stack Mills, Charlotte A.B. Bartlett, Patrick O. Gallagher, Jillian M. Lamb, Nicole J. Daigle, Shana M. Persaud, Alicia D. Gallant, Tianqi Sheng, Jane A. MacKay, Rachel O.J. Giberson, Thomas W. Leonard, Tanya E. Moore |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
biology Alaria esculenta 010604 marine biology & hydrobiology Kelp Sporophyte Plant Science Red algae Aquatic Science biology.organism_classification Laminaria digitata 01 natural sciences Palmaria palmata Chondrus crispus Botany Mastocarpus stellatus 010606 plant biology & botany |
| Zdroj: | Phycologia. 57:1-8 |
| ISSN: | 2330-2968 0031-8884 |
| DOI: | 10.2216/17-66.1 |
| Popis: | Kelp ecology is heavily biased toward the conspicuous sporophyte stage, whereas understanding of the microscopic gametophyte remains limited. Given that kelp gametophytes are known to grow in/on other species of algae, we sought to determine if species-specific polymerase chain reaction could detect kelp gametophytes in situ from coextracted host DNA. Upon verifying our molecular results, we also assessed distributional patterns of the kelp gametophytes according to site, host species, and vertical placement in the intertidal zone. We sampled Chondrus crispus, Mastocarpus stellatus, and Palmaria palmata (Florideophyceae) at Wallace Cove, New Brunswick, Canada, on 13 September 2016, where kelp sporophytes were abundant, and at an adjacent location without obvious sporophyte presence, L'Etete, on 26 September 2016. Species-specific primers were used to assess the presence of Alaria esculenta and Laminaria digitata DNA from coextracted red algal DNA. We successfully amplified kelp DNA from the host ... |
| Databáze: | OpenAIRE |
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