Spray-dried powder ofBacillus megateriumfor control of rice sheath blight disease: Formulation protocol and efficacy testing in laboratory and greenhouse
| Autor: | Ashara Pengnoo, H. Viernstein, A. Chumthong, Ruedeekorn Wiwattanapatapee, Mana Kanjanamaneesathian |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Physiology 030106 microbiology 01 natural sciences Endospore Rhizoctonia solani 03 medical and health sciences food Skimmed milk Genetics Solubility Mycelium Bacillus megaterium Aqueous solution biology Inoculation Chemistry fungi food and beverages biology.organism_classification Horticulture Agronomy Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Cereal Research Communications. 44:131-140 |
| ISSN: | 1788-9170 0133-3720 |
| DOI: | 10.1556/0806.43.2015.034 |
| Popis: | A spray-dried powder containing Bacillus megaterium was developed and tested for control of rice sheath blight disease in the greenhouse. The formulation consisted of 20 ml of an endospore suspension of B. megaterium, 20% w/v of skim milk powder and 1.25% w/v of polyvinyl pyrrolidone k-90, that were mixed and spray dried at 120 °C. The powder displayed good physical characteristics, such as a low-moisture content and a high solubility in water. Bacterial viability in the powder was 3.5±0.1 × 1011 cfu/g after production and remained relatively stable (at 2.2±0.1 × 1010 cfu/g) after 12 months of storage at room temperature. In the laboratory, a 0.1% (w/v) aqueous solution of the formulation was effective in inhibiting the mycelia growth of Rhizoctonia solani (98.5±0.1% inhibition). Under greenhouse conditions, a 0.1% (w/v) aqueous solution applied by either spraying 1 day before inoculating R. solani or spraying 1, 7 and 15 days after inoculation of rice plants with R. solani was more effective in suppressi... |
| Databáze: | OpenAIRE |
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