Quantitative Measurements of Modified Thymine Residues in DNA Chains Formic Acid Hydrolysis

Autor:	R. Téoule, A. Guy
Rok vydání:	1987
Předmět:	Formic acid Photochemistry Biochemistry Thymine Hydrolysis chemistry.chemical_compound Residue (chemistry) chemistry Polynucleotide Helix Radiolysis Polymer chemistry Genetics DNA
Zdroj:	Nucleosides and Nucleotides. 6:301-305
ISSN:	0732-8311
DOI:	10.1080/07328318708056209
Popis:	Mild formic acid hydrolysis at 90°C is a convenient method for releasing thymine modified residues which are formed upon gamma irradiation of DNA in oxygen-free solution from a polynucleotide chain backbone. The modified thymine residues are further isolated by chromatography and measured. The double helix prevents to some extent the attack of the thymine residue by the transient species OH, H and e provided by water radiolysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7f7f8411e0ed4e80fb8e6de63ebf36f5 https://doi.org/10.1080/07328318708056209 Zobrazit plný text záznamu