Dual-precipitation method evaluated for determination of high-density lipoprotein (HDL), HDL2, and HDL3 cholesterol concentrations

Autor:	P Keane, Howard G. Parsons, V C Dias, N D Boyd
Rok vydání:	1988
Předmět:	Very low-density lipoprotein Chromatography Cholesterol Biochemistry (medical) Clinical Biochemistry Polyethylene glycol chemistry.chemical_compound High-density lipoprotein chemistry PEG ratio lipids (amino acids peptides and proteins) Centrifugation Phosphotungstic acid Lipoprotein
Zdroj:	Clinical Chemistry. 34:2322-2327
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/34.11.2322
Popis:	We evaluated a dual-precipitation method for determining cholesterol in high-density lipoprotein (HDL) and its subfractions HDL2 and HDL3. After total HDL was isolated by precipitation of very-low-density (VLDL) and low-density (LDL) lipoproteins with polyethylene glycol (Mr 8000), HDL2 was isolated from total HDL by precipitation with dextran sulfate (Mr 15,000), leaving HDL3 in the supernate. Concentration of total HDL cholesterol after precipitation of VLDL and LDL with PEG showed significant proportional and constant biases of -3.8% and 0.04 mmol/L, respectively, when compared with a phosphotungstic acid-based comparison method, although results by the two methods were correlated highly (r = 0.99, P less than 0.001). HDL2 and HDL3 cholesterol concentrations measured with the present technique were not different from those obtained by density-gradient ultracentrifugation or by combined precipitation-ultracentrifugation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7f7d5941729eca9745df6e55c2568f79 https://doi.org/10.1093/clinchem/34.11.2322 Zobrazit plný text záznamu