Overexpression of an Oil Radish Superoxide Dismutase Gene in Broccoli Confers Resistance to Downy Mildew

Autor:	Cai-Ming He, Ming Jiang, Li-xiang Miao
Rok vydání:	2012
Předmět:	biology Agrobacterium food and beverages Raphanus Plant Science Plant disease resistance biology.organism_classification Molecular biology Superoxide dismutase Transformation (genetics) Botany biology.protein Hyaloperonospora parasitica Downy mildew Brassica oleracea Molecular Biology
Zdroj:	Plant Molecular Biology Reporter. 30:966-972
ISSN:	1572-9818 0735-9640
Popis:	Superoxide dismutases (SOD) play important roles in plant disease resistance. In this study, a manganese superoxide dismutase gene, designated RsrSOD, was isolated from oil radish (Raphanus sativus var. raphanistroides). RsrSOD was 696 bp in length predicted to encode 231 amino acids. The deduced amino acid sequence contained four putative Mn-binding sites. Under the control of the CaMV35S promoter, RsrSOD was introduced into broccoli (Brassica oleracea var. italica) via Agrobacterium tumefaciens-mediated transformation. Six transgenic lines were obtained out of 35 independent shoots. Both gene expression and enzyme activity of SOD increased significantly in transgenic lines when challenged with Hyaloperonospora parasitica. Three lines, L19, L23, and L25, exhibited the highest resistance against downy mildew with disease symptoms restricted completely. These highly resistant lines would serve as good broccoli breeding materials.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7f4d24c91327b639cb8a093efdb85484 https://doi.org/10.1007/s11105-011-0407-1 Zobrazit plný text záznamu Plný text ve formátu PDF