Rapid Detection Method for Enteroaggregative Escherichia coli Using Simple Clump Formation and Aggregative Assay

Autor:	Chowdhury Rafiqul Ahsan, Yoshimitsu Otomo, Miyuki Fujioka
Rok vydání:	2013
Předmět:	Rapid identification Persistent diarrhea Enteroaggregative Escherichia coli Glass slide Pcr assay medicine General Medicine Biology medicine.disease_cause Rapid detection Escherichia coli Microbiology
Zdroj:	Advances in Microbiology. :552-556
ISSN:	2165-3410 2165-3402
DOI:	10.4236/aim.2013.38074
Popis:	Enteroaggregative Escherichia coli (EAggEC) strains cause the persistent diarrhea in infants and compromised hosts in developing countries. These strains are currently defined as E. coli that adheres to HEp-2 cells in an aggregative adherence (AA) pattern. In this study, we compared 4 different rapid methods for the detection of EAggEC using a PCR assay, clump formation test, glass slide adherence assay, and the HEp-2 cell adherence assay. Out of 683 E. coli strains isolated from diarrheal stool samples, we detected 17 aggR and/or clump-positive strains, and identified 2 aggR-positive, clump-negative strains and 2 aggR-negative, clump-positive strains. All the aggR positive and clump positive strains also showed positive results in glass slide adherence and HEp-2 cell adherence assays. From all these results, we suggest the following procedure for the rapid identification of EAggEC strains: first, screen E. coli strains with the clump formation test and subsequently perform the glass slide adherence assay to observe AA for confirmation.
Databáze:	OpenAIRE
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