Purification of three cellulases from the xylophageous larvae of Ergates faber (Coleoptera:Cerambycidae)
| Autor: | C. Chararas, R. Eberhard, J.E. Courtois, F. Petek |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
chemistry.chemical_classification
Molecular mass biology media_common.quotation_subject fungi Insect Cellulase Cellobiose biology.organism_classification Biochemistry Electrophoresis chemistry.chemical_compound Enzyme chemistry Insect Science biology.protein Molecular Biology Bacteria media_common Glucan |
| Zdroj: | Insect Biochemistry. 13:213-218 |
| ISSN: | 0020-1790 |
| DOI: | 10.1016/0020-1790(83)90086-0 |
| Popis: | Three different homogeneous cellulases were isolated by ultrogel chromatography and preparative electrophoresis from the gut of larvae of Ergates faber (Coleoptera:Cerambycidae). Molecular weights (25,000 for A, 57,000 for B and 70,000 for C), optimum pH values and thermolabilities are different. Enzyme A is a 1,4-β- d -glucan cellobiohydrolase (EC 3.2.1.91) inactive on cellobiose and o- nitrophenyl β-glucoside. The two others are enzymes of wide specificity, splitting the β-glucosidic linkages of the five substrates tested. During the early phases of reaction the three cellulases can act synergically. The cellulolytic activity of this insect results from a combination of enzymes, as is the case for bacteria and fungi. |
| Databáze: | OpenAIRE |
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