Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle
| Autor: | Masatoshi Suzuki, Tohru Hosoyama, Dan Krakora, Michael G. Meyer |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Myogenesis Cellular differentiation Basic fibroblast growth factor Skeletal muscle Cell Biology General Medicine Biology Cell biology chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Epidermal growth factor Internal medicine medicine Myocyte Stem cell Progenitor cell |
| Zdroj: | Cell Biology International. 37:191-196 |
| ISSN: | 1065-6995 |
| DOI: | 10.1002/cbin.10026 |
| Popis: | Skeletal muscle progenitor cells (SMPCs) are considered one of the most valuable cells for cell-based therapy targeting skeletal muscle. However, an efficient protocol for isolating and maintaining human myogenic progenitors in vitro has not been fully established. In this study, we demonstrate that human myogenic progenitors can be expanded and proliferated from human fetal muscles. Human SMPCs were prepared from fetal hind limb muscles and induced to proliferate as free-floating spheres termed myospheres in the medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Both myogenic progenitors and myoblast populations from human fetal muscles were effectively propagated in myospheres and passaged by a mechanical chopping. After expanding these spheres in culture, we tested whether myogenic progenitor cells can differentiate into multinucleated myotubes. The myospheres were dissociated, plated down on coverslips and cultured in the medium for terminal differentiation. We could confirm that the plated cells formed well-developed, multinucleated myotubes. This culture method using myospheres is an effective protocol to isolate and maintain SMPCs from human fetal skeletal muscles in culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text