| Autor: |
Li Zhang, Qian Zhang, Xuehui Li, Zhen Liu, Yuou Teng, Huan Liu, Peng Yu, Tingshen Li, Chaoran Yao |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Lecture Notes in Electrical Engineering ISBN: 9789811048005 |
| Popis: |
E-selectin (CD62E), a transmembrane glycoprotein, is a C-type lectin that binds to the tetrasaccharide sialyl Lewis X (sLex) structure. E-selectin plays an important role in inflammation and in the adhesion of leukemia cells to endothelial cells. E-selectin can induce the interaction of specific cell–cell adhesion on the cells’ surface that can reduce the flow rate of leukocytes in the blood vessel to make inflammation worse or mediate the growth and metastasis of cancer. Actually, normal endothelial cells do not express E-selectin. However, the expression of E-selectin will be increased when endothelial cells are stimulated by inflammatory factors such as tumor necrosis factor (TNF-α), interleukin-1 (IL-β) and bacterial lipopolysaccharide (LPS). In this study, we optimized the model of THP-1 adhesion to human umbilical vein endothelial (HUVEC) includes plating density and the time requi red that E-selectin can express when inflammatory factors stimulate to endothelial cells. This paper intends to optimize the model of an adhesion between HUVEC and THP-1 through the stimulation of inflammatory factors. Meanwhile, we determined the condition for the expression of E-selectin. The results showed that the expression of E-selectin reached the peak when HUVEC was stimulated by TNF-α (20 ng/mL) for 6 h. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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