Protease-, Pectinase- and Amylase- Producing Bacteria from a Kenyan Soda Lake
| Autor: | Francis Mulaa, Patrick Wafula Okanya, Bo Mattiasson, Kevin Raymond Oluoch, Rajni Hatti-Kaul |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Protease biology medicine.medical_treatment 030106 microbiology Biomedical Engineering Biophysics Proteolytic enzymes Bioengineering biology.organism_classification Bacillus pseudofirmus Biochemistry 03 medical and health sciences 030104 developmental biology Enzyme chemistry biology.protein medicine Bacillus halodurans Food science Amylase Pectinase Bacteria Biotechnology |
| Zdroj: | The Open Biotechnology Journal. 12:33-45 |
| ISSN: | 1874-0707 |
| DOI: | 10.2174/1874070701812010033 |
| Popis: | Background:Alkaline enzymes are stable biocatalysts with potential applications in industrial technologies that offer high quality products.Objective:The growing demand for alkaline enzymes in industry has enhanced the search for microorganisms that produce these enzymes.Methods:Eighteen bacterial isolates from Lake Bogoria, Kenya, were screened for alkaline proteases, pectinases and amylases; characterized and subjected to quantitative analysis of the enzymes they produced.Results:The screening analysis ranked 14, 16 and 18 of the bacterial isolates as potent producers of alkaline proteases, pectinases and amylases, respectively. The isolates were classified into two groups: Group 1 (16 isolates) were facultatively alkaliphilicB. haloduranswhile group 2 (2 isolates) were obligately alkaliphilicB. pseudofirmus. Further analysis revealed that group 1 isolates were divided into two sub-groups, with sub-group I (4 isolates) being a phenotypic variant sub-population of sub-group II (12 isolates). Variation between the two populations was also observed in their enzymatic production profilese.g. sub-group I isolates did not produce alkaline proteolytic enzymes while those in sub-group II did so (0.01-0.36 U/ml). Furthermore, they produced higher levels of the alkaline pectinolytic enzyme polygalacturonase (0.12-0.46 U/ml) compared to sub-group II isolates (0.05-0.10 U/ml), which also produced another pectinolytic enzyme - pectate lyase (0.01 U/ml). No clear distinction was however, observed in the production profiles of alkaline amylolytic enzymes by the isolates in the two sub-populations [0.20-0.40 U/ml (amylases), 0.24-0.68 U/ml (pullulanases) and 0.01-0.03 U/ml (cyclodextrin glycosyl transferases)]. On the other hand, group 2 isolates were phenotypically identical to one another and also produced similar amounts of proteolytic (0.38, 0.40 U/ml) and amylolytic [amylases (0.06, 0.1 U/ml), pullulanases (0.06, 0.09 U/ml) and cyclodextrin glycosyl transferases (0.01, 0.02 U/ml)] enzymes.Conclusion:The facultatively alkaliphilicB. haloduransand obligately alkaliphilicB. pseudofirmusisolates are attractive biotechnological sources of industrially important alkaline enzymes. |
| Databáze: | OpenAIRE |
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