Chemotactic Movement and Attachment of Agrobacterium tumefaciens to Banana Cells and Tissues

Autor:	Serge Remy, Victor Galán Saúco, Laszlo Sagi, Juan Bernardo Pérez Hernández, Rony Swennen
Rok vydání:	1999
Předmět:	Rhizobiaceae biology Physiology Agrobacterium food and beverages Chemotaxis Plant Science Agrobacterium tumefaciens biology.organism_classification Cell biology Agar plate Transformation (genetics) Tissue culture Botany Agronomy and Crop Science Bacteria
Zdroj:	Journal of Plant Physiology. 155:245-250
ISSN:	0176-1617
DOI:	10.1016/s0176-1617(99)80014-5
Popis:	Summary For the elaboration of efficient Agrobacterium -mediated transformation of banana the initial steps in the plant-bacterium interaction were investigated. Chemotaxis of agrobacteria towards tissues was studied using a swarm agar plate system. Excised leaf, rhizome, root and in vitro proliferating tissues from different banana landraces were found to be able to elicit a positive chemotactic reaction of A. tumefaciens that could be enhanced by extensive wounding. Agrobacterium attachment was examined by UV fluorescence microscopy and scanning electron microscopy, which demonstrated the presence of bacteria individually bound or massively attached to the surface of single banana cells and tissues. It can be concluded that at least during the two early steps of interaction A. tumefaciens appears to be compatible with banana, indicating the potential for genetic transformation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7f08f6b002d62e211f6040e8dec89f51 https://doi.org/10.1016/s0176-1617(99)80014-5 Zobrazit plný text záznamu Full Text from ScienceDirect