Common Colonization Genes Profiling and BOX-PCR Based Genotyping of Streptococcus agalactiae from Pregnant Women in Tehran, Iran
| Autor: | Hanieh Banaei, Akram Sadat Tabatabaee Bafroee, Kumarss Amini |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Jundishapur Journal of Microbiology. 15 |
| ISSN: | 2008-4161 2008-3645 |
| Popis: | Background: Streptococcus agalactiae or group B Streptococcus (GBS) is a prominent cause of severe neonatal infections. Group B Streptococcus is a part of the intestinal and vaginal normal flora. Maternal colonization is recognized as the main path of GBS transmission. Group B Streptococcus is a pathobiont that changes from a non-symptomatic mucosal carriage state to a significant bacterial pathogen, causing major infections. Objectives: This study aimed to investigate the concomitant presence of major colonization genes, including ftsA, ftsB, lmb, and sfbA, and to determine the genetic relatedness of clinical GBS isolates. Methods: The GBS isolates were obtained from urinary and placental samples of pregnant women with a urinary tract infection, who were admitted to a hospital in Tehran, Iran. The presence of some major colonization factors was investigated via multiplex PCR assay. Genotyping of the isolates was performed using the BOX-PCR fingerprint technique with a BOX-A1R primer. Next, the data were analyzed using the UPGMA method and the coefficient of Jaccard in NTSYS software. Results: A total of 60 GBS isolates were examined in this study. The concomitant presence of target colonization genes was observed in all isolates. The BOX-PCR discriminated GBS isolates into six different genetic clusters at a 60% cutoff point. The majority of isolates (80%) from both clinical samples were clustered into genotypes 2, 6, and 4, while the rest (20%) were distributed equally into three different genotypes. Conclusions: Determining the colonization associated genes and genetic polymorphism in a different geographical area provides the epidemiological basis for the prevention of GBS infections in pregnant women and infants. |
| Databáze: | OpenAIRE |
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