High-performance liquid chromatographic determination of naltrexone in plasma of hemodialysis patients

Autor: Micheline Cazin, R. Azar, M. Luyckx, L. Ballester, C. Brunet, S. Bah, Thierry Dine, Pascal Odou, Jean-Claude Cazin, K. Kambia, B. Gressier
Rok vydání: 2000
Předmět:
Zdroj: Biomedical Chromatography. 14:151-155
ISSN: 1099-0801
0269-3879
Popis: A simple, sensitive, selective and reliable reversed-phase high-performance liquid chromatographic (HPLC) method with UV detection is described for the determination of naltrexone in plasma samples. Naltrexone and the internal standard, naloxone, were isolated from plasma either with a liquid-liquid extraction method using ethyl acetate or with a solid-phase extraction method using Sep-Pack C18 cartridge before chromatography. The extracts were dried under a stream of nitrogen and the samples were reconstituted in the mobile phase, then 20 microL were injected on a Waters Symmetry C18 column (5 microm particle size, 4.6 x 150 mm). The mobile phase consisted of 0.06% triethylamine (pH 2.8)-acetonitrile (92:8, v/v) pumped at 1 mL/min. The peak-area ratio versus plasma concentration was linear over the range of 10-500 ng/mL and the detection limit was less than 8 ng/mL. Quantification was by ultra-violet detection at 204 nm. The present method was applied to the determination of the plasma concentration of naltrexone in dialyzed patients. Patients (n = 8) with severe generalized pruritus received 50 mg of naltrexone orally per day for 2 weeks. The variability in the therapeutic response in treated patients required plasma concentration investigations of this opioid antagonist.
Databáze: OpenAIRE
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