A novel colorimetric and fluorometric probe for biothiols based on MnO2 NFs-Rhodamine B system
Autor: | Yingnan Liu, Min Yu, Hanyue Xue, Yuanyuan Cao, Yuhang Shui, Jing Li, Kunyi He, Li Wang, Muhammad Farooq |
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Rok vydání: | 2020 |
Předmět: |
Detection limit
010401 analytical chemistry 02 engineering and technology Glutathione 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Fluorescence Redox 0104 chemical sciences Analytical Chemistry Rhodamine chemistry.chemical_compound Fluorescence intensity chemistry Rhodamine B Environmental Chemistry 0210 nano-technology Spectroscopy Nuclear chemistry Cysteine |
Zdroj: | Analytica Chimica Acta. 1127:39-48 |
ISSN: | 0003-2670 |
Popis: | Herein, a novel bimodal ratiometric probe for sensitive and selective detection of biothiols (including glutathione (GSH), cysteine (Cys) and homocysteine (Hcys)) was constructed, which was based on the redox reaction between manganese dioxide nanoflakes (MnO2 NFs) and rhodamine (RhB) and biothiols. When MnO2 NFs was added into RhB solution, RhB was oxidized to a series of derivatives, accompanying with the colorimetric color changing from purple to light pink and fluorescence changing from red to green. In the presence of GSH, Cys or Hcys, they could reduce MnO2 NFs to Mn2+, thereby preventing the following oxidization of RhB and the corresponding color and fluorescence changes. The absorption intensity ratio and fluorescence intensity ratio showed good linear relationships with the concentrations of biothiols. The colorimetric detection limits for GSH, Cys and Hcys were 0.057 μM, 0.140 μM and 0.087 μM, respectively. And the fluorescence detection limits were 0.177 μM, 0.282 μM and 0.161 μM. More importantly, this probe was successfully applied to monitor the concentration of GSH/Cys/Hcys in human serum samples, with satisfactory recovery. Thus, this MnO2 NFs-RhB platform can potentially be a candidate for the detection of biothiols. |
Databáze: | OpenAIRE |
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