RAPD‐PCR and UP‐PCR techniques distinguish aPithomyces chartarumisolate with biocontrol capabilities againstBotrytis cinereaon grape (Vitis vinifera)

Autor:	Alison Stewart, S. L. Dodd
Rok vydání:	2003
Předmět:	food.ingredient biology food and beverages Horticulture biology.organism_classification Alternaria law.invention Microbiology RAPD food law Botany Ulocladium atrum Agronomy and Crop Science Polymerase chain reaction Pithomyces Botrytis cinerea Botrytis Ulocladium
Zdroj:	New Zealand Journal of Crop and Horticultural Science. 31:55-64
ISSN:	1175-8783 0114-0671
Popis:	Previous work identified an Ulocladium atrum (LU850) isolate with potential as biocontrol against Botrytis on grape (Vitis vinifera). The aim of this study was to find a molecular marker that would distinguish the biocontrol isolate from other isolates of the same species and closely related genera to enable subsequent monitoring of its survival, establishment, and spread in the field. DNA of 53 fungal isolates representing the three genera Ulocladium, Alternaria, and Pithomyces were screened with 10 universally primed polymerase chain reaction (UP‐PCR) primers and 28 random amplified polymorphic DNA (RAPD‐PCR) primers to screen for bands unique to the biocontrol isolate. Three RAPD‐PCR primers produced a single band unique to isolate LU850. However, resultant band patterns and subsequent more detailed morphological studies revealed the biocontrol isolate to be Pithomyces chartarum and not U. atrum. Since some isolates of P. chartarum produce the toxin sporidesmin, which is responsible for facial...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7eb4fa6b1111e1c5ec669ff5ab9d9495 https://doi.org/10.1080/01140671.2003.9514235 Zobrazit plný text záznamu