Ethidium Bromide Interaction with Poly(G)

Autor: Mikayel V. Minasyants, Marine A. Parsadanyan, Poghos H. Vardevanyan
Rok vydání: 2014
Předmět:
Zdroj: Biophysical Reviews and Letters. :239-247
ISSN: 1793-7035
1793-0480
DOI: 10.1142/s1793048014500039
Popis: In current work we investigate the interaction between polyguanylic acid and Ethidium Bromide ( EtBr ) and the changes of thermodynamic parameters due to those interactions by spectrophotometric methods. From the binding isotherms, binding constants were calculated for three different temperatures and the changes of Gibbs free energy, entropy and enthalpy for complex-formation were determined. Complex-formation with four-stranded poly(G) structure needs less conformational changes in contrast with poly(G)poly(C), and due to that, EtBr binds with 5 and not 4 nucleotides. Obtained results confirm the concept that polyguanylic acids may form four-stranded complex structures with intercalating ligand EtBr in the aqueous solution.
Databáze: OpenAIRE