Ethidium Bromide Interaction with Poly(G)
Autor: | Mikayel V. Minasyants, Marine A. Parsadanyan, Poghos H. Vardevanyan |
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Rok vydání: | 2014 |
Předmět: |
chemistry.chemical_classification
Aqueous solution Stereochemistry Intercalation (chemistry) Enthalpy Biophysics G-quadruplex Gibbs free energy Crystallography symbols.namesake chemistry.chemical_compound chemistry Structural Biology symbols Nucleotide Ethidium bromide Molecular Biology Polyguanylic Acids |
Zdroj: | Biophysical Reviews and Letters. :239-247 |
ISSN: | 1793-7035 1793-0480 |
DOI: | 10.1142/s1793048014500039 |
Popis: | In current work we investigate the interaction between polyguanylic acid and Ethidium Bromide ( EtBr ) and the changes of thermodynamic parameters due to those interactions by spectrophotometric methods. From the binding isotherms, binding constants were calculated for three different temperatures and the changes of Gibbs free energy, entropy and enthalpy for complex-formation were determined. Complex-formation with four-stranded poly(G) structure needs less conformational changes in contrast with poly(G)poly(C), and due to that, EtBr binds with 5 and not 4 nucleotides. Obtained results confirm the concept that polyguanylic acids may form four-stranded complex structures with intercalating ligand EtBr in the aqueous solution. |
Databáze: | OpenAIRE |
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