Analysis of volatile alcohols in apple juices by an electrochemical biosensor measuring in the headspace above the liquid
| Autor: | Karin Hilgert, Marcus A. Horn, Ralf Moos, Martin Hämmerle |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Detection limit
Working electrode Chromatography Metals and Alloys chemistry.chemical_element Alcohol Condensed Matter Physics Amperometry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Alcohol oxidase chemistry.chemical_compound chemistry Materials Chemistry Electrical and Electronic Engineering Hydrogen peroxide Platinum Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 158:313-318 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2011.06.026 |
| Popis: | An electrochemical biosensor was optimised for the analysis of volatile alcohols directly from the gas phase without prior absorption or pre-concentration. The sensor is based on the alcohol oxidase (Pichia pastoris) catalyzed conversion of ethanol and the amperometric detection of the generated hydrogen peroxide. Key part of the three-electrode set-up was a gas-diffusion working electrode (potential: +600 mV vs. Ag/AgCl) that consisted of a porous Teflon membrane coated with a thin platinum layer. Headspace samples were analysed for alcohols and used to derive alcohol concentrations in the liquid phase. The biosensor had a sensitivity of 3.43 μA/mM for ethanol, a response time of 69 s, a linear dynamic range of 0.10–30 mM, a theoretical detection limit (3 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect