Evaluation of flow cytometric enumeration of foetal erythrocytes in maternal blood
| Autor: | W C M Janssen, J J M L Hoffmann |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Pathology medicine.medical_specialty Fetus medicine.diagnostic_test biology medicine.drug_class business.industry Hematology Monoclonal antibody Immunofluorescence Flow cytometry Andrology Red blood cell medicine.anatomical_structure hemic and lymphatic diseases Cord blood Fetal hemoglobin medicine biology.protein Antibody business |
| Zdroj: | Clinical & Laboratory Haematology. 24:89-92 |
| ISSN: | 0141-9854 |
| DOI: | 10.1046/j.1365-2257.2002.00438.x |
| Popis: | In pregnant women subject to abdominal trauma or other foetomaternal haemorrhage, foetal red blood cells containing haemoglobin-F (HbF) can be found in the circulation. Recently, a monoclonal antibody to HbF has become commercially available, enabling application of a flow cytometric immunofluorescence method for accurately determining the concentration of HbF+ red blood cells. We demonstrate that white blood cells are included in the cluster selected as red blood cells and that these white blood cells exhibit a level of autofluorescence that coincides with the fluorescence signal from HbF+ red blood cells. However, these white blood cells can be excluded from the analysis, thus preventing spuriously increased HbF+ red blood cell counts. We present the results of patient samples containing HbF+ red cells as illustrations of the technique and as a potential interference by HbF-containing cells of nonfoetal origin. Using samples spiked with cord blood, the method is exactly linear with a high coefficient of correlation (r=0.997). Furthermore, the assay has excellent precision (CV < 2.4%), a low limit of detection (0.12% HbF+ RBC), is independent of Rhesus D and can be completed within 1.5 h. This method is suitable for accurate determination of foetomaternal haemorrhage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|